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a method for detecting abasic sites in living cellsage

A Mathematical Model for DNA Damage and Repair

H. Atamna, I. Cheung, and B. N. Ames, A method for detecting abasic sites in living cells age-dependent changes in base excision repair, Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 2, pp. 686691, 2000. View at Publisher Site Google ScholarA method for detecting abasic sites in living cells Age a method for detecting abasic sites in living cellsageJan 18, 2000A biotin-containing aldehyde-reactive probe (ARP) [Kubo, K., Ide, H., Wallace, S. S. & Kow, Y. W. (1992) Biochemistry 31, 37033708] is used to measure AP sites in living cells. ARP penetrates the plasma membrane of cells and reacts with AP A method for detecting abasic sites in living cells age a method for detecting abasic sites in living cellsageJan 01, 2000In this paper, we describe a method for biotinylating AP sites in live cells and then quantifying the biotin in the isolated DNA. This method is used to determine the age-dependent changes in BER in human fibroblasts (IMR90), leukocytes isolated from human blood, and nuclei isolated from rat

A one-step method for quantitative a method for detecting abasic sites in living cellsage - core.ac.uk

(2000). A method for detecting abasic sites in living cells age-dependent changes in base excision repair. (1985). A new approach to the study of the base-excision repair pathway using methoxyamine. (2001). A new mathematical model for relative quantication in real-time RT-PCR. (1992).Abasic Article about abasic by The Free DictionaryA method for detecting abasic sites in living cells Age-dependent changes in base excision repair. Decreased short-term spatial memory and increased oxidative stress in a scopolamine--induced rat model of Alzheimer's diseaseAuthor András Horváth and Beáta G. VértessyHeat shock protein 70 enhanced deoxyribonucleic acid base a method for detecting abasic sites in living cellsageBase excision repair (BER) of DNA damage in irradiated THP1 human leukemic cells was stimulated by pretreating the cells with exogenous recombinant Hsp70. The treatment of THP1 cells with recombinant Hsp70 in cell culture promoted repair by reducing the frequency of apurinic, apyrimidinic (AP) sites in DNA before and after 1.3 Gy of radiation. However, by 30 minutes after 2.6 Gy, accelerated a method for detecting abasic sites in living cellsage

Catalytically Competent Conformation of the Active Site of a method for detecting abasic sites in living cellsage

MATERIALS AND METHODS. Model preparation. The tertiary a method for detecting abasic sites in living cellsage H., Cheung, I., and Ames, B. N. (2000) A method for detecting abasic sites in living cells age-dependent changes in base excision a method for detecting abasic sites in living cellsage Opposite base-dependent reactions of a human base excision repair enzyme on DNA containing 7,8-dihydro-8-oxoguanine and abasic sites, EMBO J a method for detecting abasic sites in living cellsageCited by 15Publish Year 2007Author H. Fai Poon, Laila Abdullah, Jon Reed, Sarah M. Doore, Cyndi Laird, Venkat Mathura, Michael Mullan, a method for detecting abasic sites in living cellsageExpression and Functional Relevance of ANXA1 in a method for detecting abasic sites in living cellsageIntroductionMaterials and MethodsResultsDiscussionConclusionHypopharyngeal squamous cell carcinoma (HSCC) is a head and neck tumor with a high degree of malignancy and poses a serious threat to human life and health. Most patients with HSCC are diagnosed at advanced stages and 6080% of HSCC patients have lymph node (LN) metastases at initial treatment.13 With advances in diagnosis and treatment, the primary tumor control of HSCC has reached a satisfactory level, yet the 5-year survival rate ranges only between 1350%.46 Numerous studies haSee more on dovepressThe DNA repair enzyme MUTYH potentiates cytotoxicity of a method for detecting abasic sites in living cellsageHigher expression of the human DNA repair enzyme MUTYH has previously been shown to be strongly associated with reduced survival in a panel of 24 human lymphoblastoid cell lines exposed to the alkylating agent N-methyl-N-nitro-N-nitrosoguanidine (MNNG). The molecular mechanism of MUTYH-enhanced MNNG cytotoxicity is unclear, because MUTYH has a well-established role in the repair of a method for detecting abasic sites in living cellsageCited by 188Publish Year 2001Author Lawrence J Marnett, John P PlastarasBase Excision Repair as a Therapeutic Target in Colon a method for detecting abasic sites in living cellsageThe excision of these aberrant bases from DNA by glycosylases results in the formation of AP sites. MX has reactivity with the free aldehyde group at the abasic site to form a MX-AP complex (12, 13). The MX-adducted AP sites are refractory to cleavage of APE, perhaps by stabilizing the phosphodiester bonds adjacent to the AP site (12, 13). In the present studies, we demonstrate that MX induces the reduction of AP sites

Cited by 188Publish Year 2001Author Lawrence J Marnett, John P PlastarasClonogenicity of human leukemic cells protected from cell a method for detecting abasic sites in living cellsage

Pretreatment of human leukemia THP-1 cells with heat shock protein Hsp70 (Hsp70) protected them from the cell-lethal effects of the topoisomerase II inhibitor, lucanthone and from ionizing radiation. Cell viability was scored in clonogenic assays of single cells grown in liquid medium containing 0.5% methyl cellulose. Colonies were observed and rapidly scored after staining with the a method for detecting abasic sites in living cellsageCited by 248Publish Year 2008Author Nathan J. O'Callaghan, Varinderpal S. Dhillon, Philip Thomas, Michael FenechEndogenous DNA damage and mutation Trends in GeneticsAP sites are hydrolyzed by AP endonuclease then the deoxyribosyl-5-phosphate group is removed by the dRP-lyase activity of DNA polymerase . AP sites are detected by reaction of the hydroxylamine moiety of the ARP reagent (red) to form a stable oxime, which Cited by 344Publish Year 2000Author Hani Atamna, Ivana Cheung, Bruce N. AmesA Method for Detecting Abasic Sites in Living Cells Age a method for detecting abasic sites in living cellsageabasic sites in living cells in base excision repair ilar and Cell Biology, University of California, Berkeley, CA 94720-3202 arise In this paper, we describe a method for biotinylating AP sites R) of in live cells and then quantifying the biotin in the isolated DNA. ARP) This method is used to determine the age-dependent changes in

Cited by 344Publish Year 2000Author Hani Atamna, Ivana Cheung, Bruce N. AmesDirect detection and quantification of abasic sites for in a method for detecting abasic sites in living cellsage

Here we describe the development of a simple and reproducible method to measure absolute telomere length. a method for detecting abasic sites in living cellsage to minimize oxidative damage to DNA, which may alter the efficiency of the PCR if abasic sites are generated . The initial high temperature lysis and proteinase K a method for detecting abasic sites in living cellsage A method for detecting abasic sites in living cells age-dependent a method for detecting abasic sites in living cellsageCited by 344Publish Year 2000Author Hani Atamna, Ivana Cheung, Bruce N. Ames 5 minsA method for detecting abasic sites in living cells Age a method for detecting abasic sites in living cellsageJan 01, 2000A biotin-containing aldehyde-reactive probe (ARP) [Kubo, K., Ide, H., Wallace, S. S. & Kow, Y. W. (1992) Biochemistry 31, 3703-3708] is used to measure AP sites in living cells. ARP penetrates the plasma membrane of cells and reacts with AP sites in DNA to form a stable ARP-DNA Cited by 344Publish Year 2000Author Hani Atamna, Ivana Cheung, Bruce N. Ames 9 minsA method for detecting abasic sites in living cells Age a method for detecting abasic sites in living cellsageJan 18, 2000In this paper, we describe a method for biotinylating AP sitesin live cells and then quantifying the biotin in the isolated DNA.This method is used to determine the age-dependent changes inBER in human fibroblasts (IMR90), leukocytes isolated fromhuman blood,

Endogenous DNA damage and mutation - cell

In humans, approximately 107 cells divide per second. Estimates suggest that spontaneous mutations arise in about a third of those cells. These mutations arise as mistakes in DNA replication and when DNA polymerases copy damaged templates. The latter result from chemical hydrolysis of nucleoside bases or by reaction of DNA with electrophiles or reactive free radicals generated during a method for detecting abasic sites in living cellsageFrom Click to Fenton chemistry for 5bromo2 a method for detecting abasic sites in living cellsageAdditional methods to introduce DNA strand breaks for BrdU incorporation detection include ultraviolet light 56, 57 (DNA photolysis). Two disadvantages of DNA photolysis are the need of transilluminators or sensitizing agents such as Hoechst 33342 and the poor reliability of UV lamps, as compared to approaches like Fenton or click chemistry.Improving image analysis in 2DGE-based redox proteomics Recent advances in redox proteomics have provided significant insight into the role of oxidative modifications in cellular signalling and metabolism. At present, these techniques rely heavily on Western blots to visualize the oxidative modification and corresponding two dimensional (2D) gels for detection of total protein levels, resulting in the duplication of efforts.

Molecular level damages of low power pulsed laser a method for detecting abasic sites in living cellsage

Abstract Aim To study the molecular level damages in a marine bacterium, Pseudoalteromonas carrageenovora, exposed to low power pulsed laser radiation from an Nd:YAG laser. Methods

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